Current research work describe rapid high performance thin layer chromatographic determination of Artesunate and Mefloquine form its combined pharmaceutical formulation. The mention drugs were spotted on silica gel F254 TLC plates under pure nitrogen stream by Linomat TLC spotter. Separation was carried out by using methanol, toluene, Ethyl acetate, and glacial acetic acid as mobile phase in ratio of 1:4.5:4:0.1 v/v/v/v. Developed TLC plates were scanned by CAMAG TLC scanner and detection was carried out at 235 nm. Rf value of separated drugs was found to be 0.235 and 0.537 for Artesunate and Mefloquine respectively. The developed method was validated as per ICH guidelines by studying various validation parameters like Accuracy, Precision, Robustness, LOD, LOQ and solvent stability. The developed and validated method was successfully applied for determination of Artesunate and Mefloquine from its combined pharmaceutical formulation.
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